altered of microrna expression level in oligospermic patients
نویسندگان
چکیده
background: microrna (mirna) is small endogenous, single strand rna molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. mutation and/or aberrant expression of mirnas have been associated with progression of various disorders, including infertility. objective: the purpose of this research was to study the estrogen receptor beta (erβ), hsa-mir-21 and, hsa-mir-22 expression level in oligospermic infertile and control fertile men and correlation between them. materials and methods: in this study, the change in mir-21, mir-22 expression and their common target gene (erβ) expression levels were evaluated in oligospermic infertile men (n= 43) compared with 43 age matched healthy control by real-time pcr methods. results: expression analysis by qrt-pcr test on mirna have identified that mir-21, mir-22 levels were significantly higher than those in normal controls (p<0.0001) and erβ expression level significantly decreased in comparison with the normal group (p<0.0001). conclusion: our study showed that mir-21 and mir-22 are indirectly involved in spermatogenesis by regulating of the estrogen receptor and might have a diagnostic and prognostic value in men infertility
منابع مشابه
Altered of microRNA expression level in oligospermic patients
BACKGROUND MicroRNA (miRNA) is small endogenous, single strand RNA molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. Mutation and/or aberrant expression of miRNAs have been associated with progression of various disorders, including inf...
متن کاملAltered microRNA expression in patients with non-obstructive azoospermia
BACKGROUND MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility. METHODS In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were perfor...
متن کاملAltered MicroRNA expression in cervical carcinomas.
PURPOSE MicroRNAs (miRNA) are small noncoding RNAs that are 18 to 25 nucleotides in length; they regulate the stability or translational efficiency of target mRNAs. Emerging evidence suggests that miRNAs might be involved in the pathogenesis of a variety of human cancers. EXPERIMENTAL DESIGN In this study, we profiled miRNA expression in 10 early stage invasive squamous cell carcinomas (ISCC)...
متن کاملAltered microRNA expression in human heart disease.
MicroRNAs are recently discovered regulators of gene expression and are becoming increasingly recognized as important regulators of heart function. Genome-wide profiling of microRNAs in human heart failure has not been reported previously. We measured expression of 428 microRNAs in 67 human left ventricular samples belonging to control (n = 10), ischemic cardiomyopathy (ICM, n = 19), dilated ca...
متن کاملAltered expression of cell cycle regulators in adult T-cell leukemia/lymphoma patients
Abstract Background: Adult T-cell leukemia/lymphoma (ATLL) is caused by human T-cell lymphotropic virus type-1 (HTLV-1). HTLV-1 oncogenes can induce malignancy through controlled gene expression of cell cycle checkpoints in the host cell. HTLV-I genes play a pivotal role in overriding cell cycle checkpoints and deregulate cellular division. In this study, we aimed to determine and comp...
متن کاملCan Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis?
Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. On the other hand, DNA damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with DNA fragmentation and chromatin abnormalities in human ejaculates is well documented, ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of reproductive biomedicineجلد ۱۲، شماره ۱۰، صفحات ۶۸۱-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023